【摘要】 根据起源地域、类群、物种以及形态特征的差异,从海南大学苦丁茶研究所种质资源圃中选取15份冬青属苦丁茶种质材料进行过氧化物酶同工酶酶谱分析,并从冬青属5个物种中各取1份种质材料进行酯酶同工酶酶谱分析。实验结果表明:1 冬青属苦丁茶其不同物种之种质材料的过氧化物同工酶酶谱和酯酶酶谱均显示出明显的物种间差异;2 过氧化物酶同工酶在冬青属的5个供试物种的所有不同种质材料中均存在一条共同的谱带(PA3),该谱带可视为冬青属苦丁茶的特征谱带;3 不同地域来源的苦丁茶冬青种质材料其过氧化物酶酶谱显示出差异,而同一地域内(或同一类群内)苦丁茶冬青的不同种质材料,其过氧化物酶同工酶酶谱则基本相同;4 酯酶酶谱特征显示,冬青属内不同物种的苦丁茶其酯酶同工酶多态性显著。以上研究结果初步显示,过氧化物酶同工酶和酯酶同工酶之酶谱分析结果可以作为判断冬青属苦丁茶种质材料的起源地域、遗传差异、亲缘关系以及种级水平的分类鉴定的参考依据之一。更多还原

【Abstract】 According to primary statistics, there are more than 250 species of substituting-for-tea beverage plants in China (including subspecies, varieties and forms) Of all these substituting for-tea-beverage plants, 30 species are called as "Kudingcha" in the different parts of China. There are at least 4 species called and used as "Kudingcha" in Holly of China. They are Ilex kudingcha CJTseng, I latifolia Thunb, I cornuta Lindl and I pentagona SKChen, YX Feng et CF Liang This paper is a preliminary report on the isoenzyme analysis of Kudingcha15 germplasm materials of Kudingcha, from the germplasm bank of The Kudingcha Research Institute of Hainan University, were selected for the band analysis of peroxidase isoenzyme, and 5 germplasm materials from the 5 species in Holly were selected for the band analysis of esterase isoenzyme The experimental results are as follows:1 The bands of peroxidase isoenzyme and that of esterase isoenzyme showed significant species differences in the germplasm materials of different Kudingcha species in Holly2 There was a common band (PA3) of peroxidase isoenzyme in all the germplasm materials of the 5 test Kudingcha species in Holly, which can be considered as the characteristic band of these species3 The peroxidase isoenzyme bands of the germplasm materials of Ilex kudingcha CJ Tseng from the differentoriginating regions showed region differences; and that of the different germplasm materials of I kudingcha CJ Tseng from the same originating region (or in the same Taxon) were basically the same 4 The band characteristic of esterase isoenzyme showed that the polymorphism of the esterase isoenzyme of the different Kudingcha species in Holly was significantAccording to the aforementioned experimental results, the authors think that the band analysis conclusions of peroxidase isoenzyme of esterase isoenzyme can be considered as one of the reference bases for the determination of the originating region, genetic difference, relationship of the germplasm materials of Kudingcha species in Holly, as well as for the classification on the species level更多还原