The results showed the best induce medium of tissue culture for Longxi Mijiao, a special banan was 1/2 MS+6-BA 3mg/L. using medium of 1/2 MS+6-BA 4~5mg/L. The reproduction rate was more than 2.36. The culture density of about 3 cluster a bottle with 3 plants a cluster expressed obvious community effect and raised the differentiation rate.