【摘要】 用大豆总 DNA通过花粉管导入木豆品种 ICPL 870 91和 ICP70 35中 ,在 D3代出现茎色、花色、叶色、叶型等变异的植株 ,从中选择农艺性状优良的 12个单株进行全基因组原位杂交分析 ,用大豆桂品 2 4 - 2号的 DNA经地高辛标记后作为探针 ,对大豆桂品 2 4 - 2号 (供体 )、木豆 ICPL870 91(受体 )和 ICP70 35 (受体 )以及用大豆总DNA通过花粉管通道转导的木豆转化株的基因组 DNA,进行分子杂交探查。大豆探针可以探查出一些木豆转化株内有很强的杂交信号 ,比较受体、供体和转化株的原位杂交图谱 ,发现有 7个木豆转化株的 DNA与供体大豆DNA有很高的同源性片段 ,有 5个转化株的木豆 DNA与供体大豆 DNA有杂交信息但信号弱 ,表明来自供体大豆总 DNA的部分序列已整合到木豆基因组里并在其后代的性状里得到表达更多还原

【Abstract】 Total soybean DNA was transferred into pigeonpea varieties, ICPL 87091 and ICP 7035, through pollen tube channel. The D 3 transDNA plants showed variation in stem colour, flower colour, leaf colour and leaf type. Labeled with DIG High Primer DNA Labeling and Detection starter Kit II, the DNA of soybean variety Gui Pin 24-2 was used as probes to hybridize with DNAs from soybean variety Gui Pin 24-2 (donor), pigeonpea varieties ICPL 87091 and ICP 7035 (receptors) and their 12 selected transDNA progenies with better agrocharacters for whole genome DNA in situ hybridization analysis. Results showed strong DNA hybridization signal between some transDNA pigeonpea plants and soybean. Compared with the in situ hybridization performance of the donor and receptors, 7 transDNA pigeonpea progenies were clearly indicated high DNA homologous fragments, while the others showed low homologous fragments because the latter showed weak DNA hybridization signal with soybean probes. Our study results indicated some fragments of donor DNA have integrated into pigeonpea genome and expressed characters in the transDNA progenies.更多还原