【摘要】 以人用的疫苗强化免疫奶水牛和奶牛之后,分泌的免疫乳中,甲肝抗体的相对活性效价峰值维持了1周,峰值期的最大相对活性效价值在1.7左右,甲抗的相对活性效价在完成全程免疫之后,除峰值期外,维持在一个较低的水平,相对活性效价在1.2～1.5之间变动。霍乱孤菌抗体、伤寒杆菌抗体、痢疾杆菌抗体在乳汁中出现的时间要比甲抗出现的时间迟,且维持的时间很短,霍乱孤菌抗体和伤寒杆菌抗体在乳汁中能维持5周左右,痢疾杆菌抗体在乳汁中维持的时间为1周。以奶水牛免疫乳的抗体提取物进行抑菌试验,免疫乳中的抗体表现出较强的抑菌效果。当提取物中的免疫球蛋白(Ig)以终浓度为1.0mg/mL加入到霍乱孤菌和伤寒杆菌的培养液中时,能有效地抑制霍乱孤菌和伤寒杆菌的生长。更多还原

【Abstract】 kinds of human medical vaccines (to hepatitis A, cholera, dysentery and typhoid fever,respectively )were used to intensively immunize the Holland cows and milk buffalo.The phases of relative antibodies in the milk appeared after immunization were observed and some of the deference between the immune milk from Holland cows or buffalo was compared.Technological process of drawing the immune substances from the immune milk was investigated,and the bacteriostasis effects of the immune substances from the immune milk were defined.Results showed: (1) Antibodies to hepatitis A could be detected from blood of Holland cows as well as buffalo one week after immunization and it could be detected from milks produced by the two kind of animal after immunization.The activity peaking of antibodies in the buffalo milk could be seen in the 18th week (9 weeks after immunization),one week later than that in cow milk.The peaking activity values of both milks could maintain about one week.Antibodies to vibrio cholerae,to bacilli of typhoid fever or dysentery,appeared one or two weeks after immunization,with relatively low activity value.Among them,the activity of antibodies to vibrio cholerae and to bacillus of typhoid fever could last for 5 weeks,while that of antibodies to bacillus of dysentery could last for one week only. (2) Ammonium sulfate graded segregation could be used to draw antibodies from immune milk with satisfy effect.Saturation of ammonium sulfate should be changed from 35% to 25%,then to 35% again,in the rude segregation.The optimal saturation of ammonium sulfate was 33% in fine segregation.DE52 fiber column chromatography could be used to further purifying of the immune products.(3)Bacteriostasis effects to vibrio cholerae and to typhoid fever bacillus could be seen when the density of the rude product of immunoglobulin (Ig) reached 1 0 mg/mL in the culture.更多还原