【摘要】 以虎头兰杂交种"黄色热带"为材料,应用组培技术对原球茎的诱导、继代增殖和芽的分化、原球茎的切割方式、试管苗生根及移栽进行了研究,初步建立一套快繁技术体系。结果表明:以MS+6BA0.5mg/L+NAA0.2mg/L利于继代增殖,繁殖系数高达4.5,有50%原球茎直接分化成完整植株,既可保持较高的繁殖系数又可直接获得生根苗用于移栽;适宜生根的培养基为MS+NAA1.0mg/L+AC0.5%,生根率为95%;第一、二代继代培养添加香蕉匀浆汁天然复合物对虎头兰原球茎增殖有明显作用;采用纵切方式切割原球茎,有利于提高原球茎的增殖;活性炭对试管苗的生根有促进作用;苔藓是虎头兰移栽较好的基质,在适宜的温度和湿度条件下,试管苗移栽成活率可达95%。更多还原

【Abstract】 Based on the hybrid’Yellow tropic’of the Cymbidium hookerianum,the induction of protocorm,subculture and differentiation of buds,slice methods of tube seedling,rooting rate, and transplant of tube seeding were studied and finally builded up the systems of the tissue propagation techniques for the ’Yellow tropic’. The results showed:(1)MS+6-BA 05 mg/L+NAA 02 mg/L for the subculture, the multiplication rate was over 45,50% of protocorm directly differentiated into the seedling .As a result,it not only retained high propagation rate, but also acquired rooting seedling for the transplantation.(2) MS+NAA 10 mg/L+AC 05% for rooting,rooting rate was 95%.(3) The 1th and 2th secondary cultrure media supplemented with the fruit juice of banana was beneficial to the propagation of protocorm.(4) the vertical slice for protocorm was beneficial to the propagation of the protocorm.(5) Adding the activated carbon was benefical to the rooting of tube seedling. The moss was the ideal media for transplanting.With proper temperature and humidity,survival rate of translanting was about 95%.更多还原