【摘要】 目的:探讨大学生载脂蛋白E（apoE）基因多态性,及其对血脂代谢紊乱、动脉粥样硬化（AS）和冠心病（CHD）的影响。方法:对广东地区高校152名健康汉族大学生,在问卷调查的基础上,根据一、二级亲属有无CHD病史分为阳性组与对照组,采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行apoE基因分析。结果:有CHD家族史的阳性组e₄等位基因频率显著高于无CHD家族史的阴性组（P<0.05）。结论:apoE基因多态性与AS、CHD易感性密切有关,e₄可能是CHD的重要遗传因子。更多还原

【Abstract】 Objective: To investigate the effect of apolipoprotein E(apo E) gene polymorphism on blood lipids metabolism and the incidence of atherosclerosis (AS) and coronary heart disease (CHD) in college students . Methods: One hundred fifty two healthy students of Han nationality in the universities of Guangdong region were investigated by using polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP) analysis. With the help of information from questionnaire, they were divded into 2 groups according to whether or not they had positive CHD family history and then they underwent apoE genotype analysis. Results: The allele frequency of apo e4 in positive group was significantly higher than that of negative group (P<0.05). Conclusions: The apo E gene polymorphism correlated significantly with susceptibility to AS and CHD, and e4 may be an important genetic factor of CHD.更多还原