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盐酸小檗碱与脱氧核糖核酸相互作用的研究
Study on the Interaction between Berberine Hydrochloride and DNA
【摘要】 本文采用紫外光谱、荧光光谱、荧光偏振 ,热变性曲线等手段 ,对小檗碱 (BR)与小牛胸腺脱氧核糖核酸 (ct DNA)的作用方式进行了研究 ,证实了盐酸小檗碱通过嵌插方式与ct DNA相互作用的 ,并且根据荧光峰增强与BR浓度的线性关系 ,提出了BR的测定方法。
【Abstract】 The characteristics of the interaction between berberine and ct-DNA were studied by UV-Vis Spectrum, fluorescent spectrum, fluorescent polorization etc. The results show that berberine hydrochloride interacts with ct-DNA in the mode of intercalation. According to the linear relation between the fluorescence intensity and the concentration of berberine, we proposed a method of the content determination of berberine.
【关键词】 荧光光谱;
荧光偏振;
小檗碱;
小牛胸腺脱氧核糖核酸;
【Key words】 Fluoresence spectrum; Fluorescence polarization; Berberine; ct-DNA;
【Key words】 Fluoresence spectrum; Fluorescence polarization; Berberine; ct-DNA;
【基金】 山东自然科学基金资助项目
- 【文献出处】 光谱学与光谱分析 ,Spectroscopy and Spectral Analysis , 编辑部邮箱 ,2003年04期
- 【分类号】O657.3
- 【被引频次】45
- 【下载频次】397