【摘要】 [目的 ]考察微滤 -大孔树脂法精制苦参中氧化苦参碱、苦参总黄酮的效果 ,并与水提醇沉法进行了比较。[方法 ]采用 HPL C法测定氧化苦参碱的含量 ,紫外分光光度法测定苦参总黄酮的含量。 [结果 ]苦参提取液经微滤 -大孔树脂法处理后 ,其氧化苦参碱、苦参总黄酮的保留率为 78.88%和 73.4 % ,固形物去除率为 38.95 % ;经醇沉法处理后 ,氧化苦参碱、苦参总黄酮的保留率为 6 0 .7%和 5 2 .1% ,固形物去除率为 34.88%。 [结论 ]微滤 -大孔树脂法较醇沉法可更有效地保留有效成分、去除杂质。更多还原

【Abstract】 [Objective]To study the effect of microfiltration macroporous resin method in refining of oxymatrine and general flavone in sophora flavescens,and compare with alcohol sedimentation.[Methods]HPLC was applied to analyze the content of oxymatrine in the samples,UV spectrophotometry was applied to analyze the content of general flavone.[Results]In sophora flavescens decoction,the recovery ratio of oxymatrine and general flavone were 78 88% and 73 4% respectively,the whole solid was decreased by 38 95% by microfiltration macroporous resin method;and the recovery ratio of oxymatrine and general flavone were 60 7% and 52 1%,the whole solid was decreased by 34 88% by alcohol sedimentation.[Conclusion]Microfiltration macroporous resin method was more effective than alcohol sedimentation for refining oxymatrine and general flavone in sophora flavescens.更多还原