【摘要】 以发育25～30d幼胚诱导的愈伤组织为试材,采用染色体计数、RAPD和AFLP标记,对贵农5号(Rosarox-burghiiTrattcv.GuinongNo.5)及白花刺梨(R.roxburghiiTrattf.candida)不同继代次数愈伤组织的遗传稳定性进行检测。结果表明,刺梨染色体非整倍性现象较为普遍,在1～2代时即占观察数的9.43%,在第20代时为20.75%,其中亚倍体为15.09%;采用124个随机引物和54对选择性引物扩增,贵农5号在第15代能检测出变异,而第20代分别检测出1.96%和1.68%的变异标记;在白花刺梨上,共扩增出1336条清晰AFLP带,其中第15代检测出7条变异带,而第20代的变异带为2.24%。因此用于种质资源离体保存时,刺梨愈伤组织的继代数应控制在10代以内。更多还原

【Abstract】 To evaluate the genetic stability of Guinong No 5(Rosa roxburghii Tratt)and white-flower chestnut rose(R.rox-burghii Tratt f.candida S.D.Sh)callus induced from25～30d old immature embryo,cytology observation,RAPD and AFLP markers were employed.Aneuploids were commonly observed in callus of the1st/2nd subculture(9.43%),and in callus sub-cultured for20times,the varied cells counted for20.75%,out of which15.09%were hypoploids.Using124arbitrary primers and54selective primer pairs,the genetic variations were detected in callus of Guinong No 5sub-cultured for15times,1.96%of RAPDs and1.68%of AFLPs were aberrant when the callus was sub-cultured for20times;1336distinct AFLPs were scored in white-flower chestnut rose,out of which,7were polymorphic in the callus sub-cultured15times;while2.24%of the total showed variation in the20th subculture.To maintain genetic stability,the callus of chestnut rose should not be sub-cultured more than10times.更多还原