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荧光光度法测定胰腺肿瘤细胞膜上的潘生丁
Determination of Dipyridamole on PAC-1 Cell Membranes by Fluorimetry
【摘要】 测定了胰腺肿瘤细胞膜上的潘生丁在乙腈 -水溶液中的荧光光谱和强度 ,并推算出胰腺肿瘤细胞膜上核苷载体的数量。潘生丁的浓度与其荧光强度在 1.0× 10 -12 — 5 .0× 10 -5mol/ L范围内呈线性关系 ,检出限为 2 .8× 10 -13 mol/ L(1.4× 10 -4ng/ m L) ,相对标准偏差为 2 .7%。
【Abstract】 A fluorescence spectroscopy and a fluorimetry for the determination of dipyridamole on PAC-1 cell membranes in acetonitrile-water(2∶1,V/V) were studied.The concentration range for the determination of dipyridamole was in the range of 1.0× 10 -12-5.0×10 -5mol/L with detection limit of 2.8×10 -13mol/L(1.4×10 -4ng/mL) and RSD of 2.7%.The number of nucleoside transporters on each PAC-1 cell membrane was calculated.
【关键词】 潘生丁;
荧光光度法;
胰腺肿瘤细胞膜;
核苷载体;
【Key words】 Dipyridamole; Fluorimetry; PAC-1 Cell Membranes; Nucleoside Transporter.;
【Key words】 Dipyridamole; Fluorimetry; PAC-1 Cell Membranes; Nucleoside Transporter.;
- 【文献出处】 光谱实验室 ,Chinese Journal of Spectroscopy Laboratory , 编辑部邮箱 ,2003年06期
- 【分类号】R735.9
- 【下载频次】40