【摘要】 本文用 2 - [6-双乙酸基 - 5 - ( 2 -双乙酸基 ) - 5 -甲苯氧乙氧基 ]- 2 -苯骈呋喃基 - 5 -恶唑羧酸五钾盐 ( Fura- 2 )建立了测定人外周血中性粒细胞内游离钙浓度的方法 ,并对一些测试条件进行了探讨。测定标本内中性粒细胞数以大约 10 6个 /m L为宜 ,在离心分离出中性粒细胞悬液时 ,标本制备好需静置 0 .5 h后才能上机测试 ,否则振荡形成的剪切应力将增大细胞内游离钙的浓度 ,p H偏离 7.4和受损细胞会极大增加细胞内游离钙的浓度。结果表明 ,正常人外周血中性粒细胞游离钙浓度为 3.95± 0 .76μg/L。对中性粒细胞内游离钙的测定有助于了解其功能状态。更多还原

【Abstract】 The concentration of the cytosolic free calcium in peripheral blood neutrophil cells were measured using Fura-2 method,and the experimental conditions were studied.It was found that about 106 cells per milliliter of measured samples were suitable to the analysis.The shear stress can enhance the concentration of cytosolic free calcium in the cells,and pH and injuring cells affected the concentration of the cytosolic free calcium in the cells.The free calcium concentration is 3.65±0.76μg/L in human neutrophil cell.The results are helpful to find out the functional state of those cells.更多还原