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兔髂骨骺板软骨细胞体外构建骺板样软骨组织

Reconstruction of Growth-plate in Vitro from Iliac Chondrocytes of Rabbits

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【作者】 王金成尹飞郭丽段德生高忠礼

【Author】 Wang Jincheng,Yin Fei,Go Li,et al.Department of Orthopedics,Third Teaching Hospital,Norman Bethune Medical School of Jilin Univercity.Changchun,130031

【机构】 吉林大学中日联谊医院骨科吉林大学公共卫生学毒理教研室吉林大学中日联谊医院骨科 长春市130031长春市130031长春市130031

【摘要】 目的 利用组织工程学技术体外构建骺板样软骨组织。方法 从 4~ 5周龄兔髂骨骺板软骨处获取软骨细胞 ,在离心管内轻微离心后 ,体外培养。行组织学观察。结果 培养至第 7天时 ,细胞呈现定向分化 ,形态与体内骺板软骨细胞相类似 :肥大软骨细胞体积较大、呈圆形或椭圆形 ;增殖、成熟软骨细胞体积小 ,呈圆形或扁圆形 ;细胞周围充满大量的细胞外基质。这些不同分化阶段的细胞形成了分化区带 ,肥大软骨细胞位于上侧 ,增殖、成熟细胞位于中间 ,其次是散在静止软骨细胞。培养第 14天 ,分化区带更加明显 ,增殖、成熟细胞和肥大软骨细胞呈现纵向定向排列。培养第 2 1天 ,组织表面出现膜样的结构。结论 体外构建的骺板样软骨组织与天然骺板的组织学形态极为相似。从髂骨处骺板处获取肥大软骨细胞进行体外构建骺板软骨材料 ,更具有临床实用性

【Abstract】 Objective To reconstruct the growth plate like tissue in vitro from iliac chondrocytes of rabbits.Methods Chondrocytes isolated from growth plate iliac cartilage of 4 to 5 week old New Zealand rabbits were centrifuged,then the cell pellet was incubated in centrifuge tubes.Results The cultures reorganized into growth plate like tissue which could be seen 7~21 days after cell seeking.The growth plate like tissue,histologically,was organized horizontally into four cellular zones containing resting,proliferation,maturing and hypertrophic cell and longitudinally into cellular columns.The hypertrophic cells in the upper were large in size and round or oval in shape,the proliferating and the mature chondrocytes in the lower were small in size and spherical or elongated in shape.These chondrocytes were surrounded by an extensive matrix.Conclusion The growth plate like tissue reorganized in vitro from iliac chondrocytes is analogous to the in vivo histological structure of growth plate.

【基金】 国家自然科学基金资助项目 (39970 741 )
  • 【文献出处】 骨与关节损伤杂志 ,The Journal of Bone and Joint Injury , 编辑部邮箱 ,2003年05期
  • 【分类号】R329.2
  • 【被引频次】8
  • 【下载频次】64
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