【摘要】 目的　探讨口腔鳞癌中p16与p5 3基因表达特征和临床病理意义。 方法　对 31例口腔鳞癌组织采用免疫组化方法检测P16及P5 3蛋白表达。结果　 31例口腔鳞癌中P16蛋白阳性表达 17例 ,阳性率 5 4 .8% ,P5 3阳性表达 16例 ,阳性率 5 1.6 %。P16在鳞癌Ⅰ级中阳性表达 14例 (6 3.6 % ) ,Ⅱ级中阳性 1例 (33.3% ) ,Ⅲ～Ⅳ级中阳性 2例 (33.3% )。P5 3在鳞癌Ⅰ级病例中阳性 10例 (4 5 .5 % ) ,Ⅱ级中 1例 (33.3% ) ,Ⅲ～Ⅳ级中 15例 (83.3% )。P16蛋白阳性表达率随病理恶性程度增高而降低 ,但无统计学意义。结论　P5 3蛋白阳性表达率随病理恶性程度升高而增高 ,提示p5 3基因与口腔鳞癌细胞分化有相关性更多还原

【Abstract】 Objective To detect the clinicopathological significance of p16 and p53 gene expression in oral squamous cell carcinomas. Methods The expression of P16 and P53 were examined in 31 cases of squamous cell carcinomas with immunohistochemical techniques. Results The positive staining of P16 appeared in cell nuclei and cytoplasmas, while P53 appeared only in cell nuclei. The positive rate of P16 expression was 54.8%(13/17). 16 of 31(51.6%) samples expressed P53 protein, among which, squamous cell carcinoma grade Ⅰ was 45.5%(10/12), grade Ⅱ 33.3%(1/3), grade Ⅲ～Ⅳ 83.3%(5/6), and there was a significant difference between grade Ⅰ and grade Ⅲ～Ⅳ. Conclusion There was no correlation between the expression of p16 and p53 genes, so further studies examining mechanisms of p16 in activation are needed to clarify the relationship between p16 and p53 in oral squamous cell carcinoma.更多还原