【摘要】 通过聚赖氨酸修饰将微过氧化物酶 -1 1 ( MP-1 1 )固定在金电极表面 ,制备成 MP-1 1修饰电极 .修饰在电极表面上的 MP-1 1的血红素活性中心与电极之间可进行直接的电子传递反应 ,其氧化还原式电位为-0 .3 9V.该修饰电极对氧的还原具有电催化活性 .当 MP-1 1与咪唑发生轴向配位反应时 ,其氧化还原式电位发生负移 ,此时对氧的还原不再具有电催化活性 .更多还原

【Abstract】 MP-11 was immobilized on the surface of the poly-L-lysine modified gold electrode. Direct electron transfer between the heme and the electrode was observed. The formal redox potential for MP-11 immobilized at the gold electrode is -0.39 V in the phosphate buffer solution(pH=7.0). The MP-11 modified electrode possesses the good electrocatalytic activity for the reduction of O 2. When imidazole becomes the sixth ligand, the formal redox potential of MP-11 would shift to the negative direction and the electrocatalytic activity of MP-11 for the reduction of O 2 is significantly inhibited.更多还原