【摘要】 以带有羧基的酸性药物吲哚美辛为模板分子、碱性的 4 -乙烯基吡啶为功能单体 ,采用水溶液微悬浮聚合法制备了用于色谱分离的微米级分子印迹微球 .详细讨论了流动相中缓冲溶液的 p H值对吲哚美辛在MIMs柱上的容量因子 (k′)、分离因子 (α)和印迹因子 (β)的影响 .通过 MIMs柱对吲哚美辛和 4 -氨基吡啶(4 -AP)的保留行为的比较 ,证明以 4 -乙烯基吡啶为功能单体制得的 MIMs对吲哚美辛的识别作用 ,主要靠吡啶环上氮原子与吲哚美辛羧基之间的离子键相互作用 ,以及吡啶环与模板分子之间的π-π相互作用 .更多还原

【Abstract】 The molecularly imprinted microspheres(MIMs) for high performance liquid chromatography were synthesized by aqueous micro-suspension polymerization. The acidic indometacin, one of the antidepressants, was used as the template and basic 4-vinylpyridine was used as functional monomer. The influences of pH of mobile phase on the capacity factor(k′), separation factor(α) and selection factor(β) of indometacin were investigated in detail. All the results showed that the MIMs exhibited specific recognition and affinity to indometacin only in pH<4.0 eluent; and the specific recognition of MIMs was lost gradually in pH>4.0 eluent. By comparing the retention behavior of indometacin and 4-aminopyridine (4-AP) on the MIMs column, it proved that the recognition of MIMs to indomeatcin synthesized by 4-vinylpyridine as a cross-linker mainly relied on the ion-pair interaction between the nitrogen atom on the pyridine ring and the carboxyl on the indometacin, and the π-π interaction between the pyridine ring and the template molecule. This was just contrary to that MIMs synthesized by methacrylic acid as the cross-linker.更多还原