【摘要】 在气液界面上研究了 α-D-甘露糖苷 -十六烷 ( MC1 6 )与 1 0 ,1 2 -二十五碳双炔酸混合单分子膜行为 ,二者具有较好的互溶性 .在疏水的玻璃衬底上用 Langmuir-Schaefer( LS)薄膜技术制备单层 MC1 6 /PDA薄膜 ,研究了这种仿生薄膜与大肠杆菌 jm 1 0 9的相互作用 .大肠杆菌 jm 1 0 9对 MC1 6 修饰的聚二乙炔 LS薄膜的吸附 ,使薄膜颜色由蓝色变为红色 ,用紫外 -可见吸收光谱可进行定量检测 .动力学研究显示比色响应值随时间的增加而增加 ,3 min内即有显著变化 ,1 5 min后趋于饱和 ,2 0 min后 CR值达到 2 8% ,进一步探讨了分子识别的动力学过程更多还原

【Abstract】 As a potential materials for optic biosensor, the surface properties of the polydiacetylene films fuctionalized with glycolipid on pure water are interesting and important. Pressure area isotherms of mixed α D mannoside hexadecyl(MC 16 ) and 10,12 pentacosadiynoic acid(PDA) monolayers were studied. The interfacial behavior at the air/water interface show that MC 16 /PDA monolayers on the water surface have a good miscibility. The interactions of MC 16 with Escherichia coli(E.coli) jm 109 were studied by rapid colorimetric transitions on the MC 16 /PDA Langmuir Schaefer (LS) films. This colorimetric transition can be quantified by UV Vis absorption spectra. The kinetic experiments show that the color change induced by the addition of E.coli reaches a plateau after 15 min, although the change becomes apparent within 3 min. Kinetics of the process was discussed. We estimate that one Escherichia coli can only bind two MC 16 molecules.更多还原