【摘要】 组装了由注射泵进样系统、微流控芯片和三温区加热器组成的流动型 PCR扩增系统 ,该系统具有扩增速度快、交叉污染小、芯片可重复使用和操作方便等特点 .优化了芯片厚度、隔热材料和流速等影响 PCR扩增的因素 .在 4 .9min内经 2 4个循环成功地扩增了浓度为 1 ng/1 0 0μL的λ-DNA( 5 0 0 bp) .更多还原

【Abstract】 A PCR amplification system comprising a syringe pump sample processor, a microfluidic PCR chip and a 3-temperature-zone heating block was developed in this work. The system is capable of performing continuous PCR amplification with a low carryover between neighboring samples. The substrate thickness and thermal insulation of the temperature control unit were optimized. A 24-cycle PCR amplification of a 500-base pair fragment from λ-DNA was successfully performed with a total reaction time of 4 9 min.更多还原