【摘要】 目的应用2215细胞株作为体外抗乙型肝炎病毒(HBV)的实验模型,在观察海珠益肝胶囊对乙型肝炎病毒e抗原(HBeAg),表面抗原(HBsAg)抑制作用的基础上,从细胞凋亡和凋亡相关基因表达影响的角度,探讨该药抗HBV的药物作用机制。方法 细胞染色采用S—P免疫组化法,通过图像分析仪测定阳性细胞染色的平均光密度,并应用流式细胞仪检测药物诱导细胞凋亡情况,观察药物对2215细胞调控蛋白Fas、Bax、Bcl-2以及对2215细胞本身凋亡诱导的影响。结果(1)海珠益肝胶囊对Fas、Bax基因蛋白的表达与正常对照组相比有显著性差异(P<0.05),即能诱导Fas、Bax的高表达;同时能下调bcl-2的表达,与正常对照组比亦有显著性差异(P<0.05)。(2)用海珠益肝胶囊处理过的2215细胞的单细胞悬液,经流式细胞仪检测其DNA含量,呈现典型的凋亡图谱。结论海珠益肝胶囊通过调节肝细胞凋亡、调控蛋白基因表达,并诱导被感染的肝细胞凋亡是其抗病毒机制之一。更多还原

【Abstract】 Objective Cell strain 2215 was used as an experimental model in vitro for anti - HBV experiment. Methods S-P immunohistochemical method and flow cytometry were used to identify the expression of bcl - 2 gene, Fas gene and Bax gene in HepC2215 cells after treatment of Haizhu Yigan capsule. Result Haizhu Yigan capsule upregulated Fas and Bax gene compared with ACV group. After treatment of Haizhu Yigan capsule, there was a sub- Cl peaks (apoptotic peak) in DNA histogram by the method of cell cycling analysis (PI - FCM). Conclusion The anti - HBV mechanism of Haizhu Yigan capsule might be associated with up - regulating the protein expression of Bax and Fax gene and down - regulating the protein expression of bcl - 2, accordingly, inducing 2215 cell apoptoeis.更多还原