【摘要】 观察了γ射线照射大鼠双侧肾脏对骨代谢的影响。6月龄雄性SD大鼠20只,随机分为对照组(n=10)和双侧肾脏γ射线照射组(n=10)。应用137Csγ射线照射装置每侧肾脏照射15Gy,剂量率为0.91Gy/min,照射后饲养3个月。收集24h尿,处死动物,收集血、肾脏、腰椎、股骨、胫骨标本。测定血肌酐、尿素氮、钙、磷、25(OH)D3、1,25(OH)2D3、PTH和尿生白、β2微球蛋白、钙、磷、PYD/肌酐等指标。肾脏标本作光镜和透射电镜观察。第1—4腰椎和右侧股骨作骨密度测定,第3腰椎和右侧股骨作成分分析,第4腰椎和左侧胫骨上段作形态计量学分析。结果表明,射线致肾小球、肾小管和肾间质病理性损伤。与假手术组比较,射线照射组血尿素氮、肌酐和尿β2微球蛋白分别升高26％(p<0.05)、9％和29.6％(p<0.05),血清钙降低4％,血清磷升高2％,尿钙增加24％,尿磷减少35％(p<0.05),血清碱性磷酸酶活性提高17％(p<0.05),血清25(OH)D3变化不明显,而1,25(OH)2D3降低54.9％(p<0.05)血清PTH升高27.3％(p<0.05),尿PYD/肌酐值增加31.7％(p<0.05),腰椎骨密度降低13.0％(p<0.05),第4腰椎骨矿盐含量降低13.1％(p<0.05),右侧股骨骨密度、干重和灰重分别降低3.5％(p<0.05)、7.6％(p<0.05)和11.6％(p<0.05),骨小梁体积、平均骨小梁厚度和结点末端比分别下降13.2更多还原

【Abstract】 The work was to observe the effects of γ-rays radiation of rat kidneys on rat bone metabolism. Ten male SD rats aged 6 months were irradiated at their kidneys with 15Gy 137Cs r-rays( 0.91 Gy/min) and were raised for 3 months after the radiation. On collecting 24h urine of rats they were sacrificed for serum, kidney, spine , femur and tibia exams. Results show that the γ-ray irradiation could induce the pathological injuries of renal glomeruli, tubules and mes-enchyme. Comparing to the control group, significant chages were found in the irradiated group in terms of their blood urea, nitrogen creatinine, urinal β-2 microglobulin, serum Ca and P, urine Ca and P, activity of serum alkaline phosphatase, 1,25(OH)2 D3, serum PTH, urine PYD/creatinine, bone mineral density(BMD) of lumbar vertebras, mineral mass of No.4 lumbar vertebra, BMD, dehydrated weight and ash weight of right femur. Marked changes were also found in bone trabe-cula volum, average bone trabecula thick and the ratio of nodes/points, and rate of mineralizatal deposition. It was concluded that renal dysfunction and metabolic bone disease might occur with the character of accelerated bone turnover and decreased bone mass.更多还原