【摘要】 研究了不同毛细管预处理方法对组胺、5 羟色胺及儿茶酚胺类神经递质电泳分离的影响,采用优化的毛细管预处理方法及电泳分离条件基线分离了组胺、5 羟色胺、去甲肾上腺素和肾上腺素。利用多巴胺和5 羟色胺在毛细管内壁吸附效应的不同,对电泳淌度极为相近的多巴胺和5 羟色胺进行了分离和鉴定。以PC12细胞为研究对象,证实了该细胞中所含大量神经递质是多巴胺,不是5 羟色胺。更多还原

【Abstract】 A systematic study on the separation efficiency and the detection sensitivity of catecholamines, histamine and 5hydroxytryptamine with differently preconditioning capillary was investigated in detail. The optimum capillary preconditionings for the separation of the several biogenic amines were explored. Histamine, 5hydroxytryptamine, norepinephrine and epinephrine were separated under the optimum conditions. The dopamine peak in PC12 cells was identified by the pH hysteresis effect in capillary electrophoresis. 更多还原