【摘要】 本文研究了在微乳液介质中,pH=10.0时,铅与邻硝基苯基荧光酮(o NPF)显色生成稳定的1∶3配合物,在598nm处摩尔吸光系数为8.74×104L·mol-1·cm-1,铅含量在0～1.2μg/mL范围内符合比耳定律。采用巯基葡聚糖凝胶分离富集,消除了共存离子的干扰。用所拟方法测定食品中的微量铅,结果令人满意。更多还原

【Abstract】 In this paper, the color reaction of lead with o-nitrophenylfluorone （o-NPF） in microemulsion has been studied. Lead （II） reacts with o-NPF to form a stable 1∶3 complex at pH 10.0. Its apparent molar absorptivity is 8.74×10⁴ L·mol^-1·cm^-1 at 598 nm. Beer’s law is obeyed in the range of 0～（1.2 μg/mL）. In order to improve the selectivity and sensitivity of the developed system in real sample analysis, sulfhydryl dextrane gel （SDG） separation/preconcentration prior to spectrophotometric analysis was employed. The method is used to determine trace amount of lead in food samples with satisfactory results.更多还原