【摘要】 用光谱电化学和电化学技术研究了硫堇和硫堇与 DNA相互作用的电化学和光谱电化学性质 ,得出了硫堇作为有机小分子能插入 DNA双螺旋结构内部的结论 ,求得了对应的动力学参数。DNA的加入量与硫堇峰电流的降低呈一次线性关系 ,可望用于 DNA浓度检测更多还原

【Abstract】 This paper reports our recent research about the electrochemical character of thionine and the interaction between thionine and DNA by electrochemistry and spectroelectrochemistry technologies. The results show that thionine can insert into the inner of the DNA bi-helix structure. The kinetics parameters, the number of transferred electrons, standard potential E 0’ and reaction rate constant are obtained by spectroelectrochemistry with results of 1.033, -0.209 V and 2.51×10 -3 s -1, respectively. Moreover, the thionine peak currents are decreased linearly with increased DNA concentration.更多还原