【摘要】 实验用金电极自组装技术构建透明质酸结合蛋白电容型免疫传感器 ,采用交流阻抗技术对电极表面生物绝缘膜进行研究。按R(RC)等效电路图对其阻抗谱进行拟合的偏差较小 ,显示实际体系接近于拟合的电路图。该传感器对 1～ 10 0mg L抗原具有良好的线性响应。构建的生物膜对于一定范围的离子强度、酸度具有良好的稳定性 ,表明其有望应用于临床检测更多还原

【Abstract】 Hyaluronan binding protein (HABP) capacitive immunosensor was fabricated with self assembled technology and studied with impedance spectroscopy in the absence of Faradaic reaction. The deviation of fitting based on R (RC) circuit was little, which indicated that the system was similar to the model. In the range of 1～100 mg/L, the immunosensor showed a good linear response to HABP antigen. Increasing the ion strength and the acidity of the electrolyte, the monolayer was stable without any pinholes. This made the immunosensor be promising in the clinical application.更多还原