【摘要】 在毛细管电色谱中 ,对浓度极稀的样品 ,通过加长进样时间 ,控制操作条件可能有效地实现溶质在柱内的富集 ,提高检测灵敏度。本文根据驰豫理论的基本原理 ,从理论上探讨了进样过程中溶质在固定相上的吸附作用对其分布的影响规律。理论和试验皆证明 :采用超过时间进样的方法 ,通过控制进样区带与运行区带中有机调节剂浓度、离子强度等条件 ,导致溶质在区带界面处的速度产生阶跃 ,能够使中性溶质仅保留在柱内较窄的区带中 ,这一过程类似于完成一次固相微萃取过程 ,能够达到较好的富集效果。对于药物安息香达到了超过 2 0 0 0倍的富集效果更多还原

【Abstract】 The effect of solute absorbed on the solid phase on its distribution in electro-chromatographic column was investigated according to relaxation theory. The distribution equation was theoretically gotten by production function. The result shows that neutral solute can be stayed in a narrow band near column head at ultra-long injecting time, indeed longer than dead-time. This process is similar to a solid-extraction. As an example,benzoin can be enriched 20000 fold with this method.更多还原