【摘要】 合成了一种新型的快速响应热敏高分子聚N 异丙基丙烯酰胺 丙烯酰胺 [P(NIP co AA) ],通过改变丙烯酰胺的含量可以改变高分子的临界溶解温度 (LCST) ,使之用于不同用途。其中 ,将相转变温度 (Ttr)在37℃的热敏高分子用于免疫分析的载体 ,建立了夹心型荧光免疫分析兔IgG的新方法。与聚N 异丙基丙烯酰胺 (PNIP)作载体相比 ,两者灵敏度相当 ,但由于相转变温度的提高 ,使得免疫反应的温度更接近于生物体的生理环境 ,并使免疫反应速率得到提高。该方法线性范围为 0～ 10 0 0 μg L ;检出限为 10 μg L。用于兔血清中兔IgG的测量 ,结果令人满意更多还原

【Abstract】 A novel temperature sensitive polymer, poly(N isopropylacrylamide co acrylamide) [P(NIP co AA)], was synthesized. Its lower critical solution temperature (LCST) could be regulated by changing the content of acrylamide for different purposes. The P(NIP co AA) with phase transition temperature ( T tr )of 37℃ was used as a separating carrier for the reactants in immunoassay and a sandwich fluorescence immunoassay for rabbit IgG was established. The sensitivity of this method was comparable to that using poly N isopropylacrylamide(PNIP) as a carrier. However, owing to the elevation of T tr of the polymer, the immunoreaction could be carried out at a temperature closer to the physiological environment of organism and the rate of immunoreaction was faster. The linear range of determination was 0～1000 μg/L and the limit of detection was 10 μg/L. This method has been applied to the determination of the rabbit IgG levels in rabbit serum with satisfactory results.更多还原