【摘要】 目的 :验证性腺激素对瘦素分泌的影响 ,寻找妊娠期瘦素峰出现的原因。方法 :用 3月龄的金黄仓鼠作为实验动物。制备雌激素及孕激素硅胶埋植管、检验其释放剂量以用于提供后续实验中持续性的高水平雌激素及孕酮 ;制备雌激素以及抑制素的抗血清、检验其生物活性 ,用于后续实验中消减体内这两种激素的生物学作用 ,观察这两种激素缺失对于瘦素分泌活动的影响 ;通过妊娠期间卵巢切除来观察该期间内性腺对于瘦素分泌活动的影响。通过对于切除性腺的动物激素埋植的方法来观察不同激素对在体时瘦素分泌的作用。结果 :金黄仓鼠于妊娠第 11天去除卵巢后瘦素水平显著下降 ,妊娠第 12天的瘦素峰消失 ;妊娠第 11天注射雌激素及抑制素抗血清对瘦素的分泌没有产生显著影响 ;与正常对照相比 ,未孕的金黄仓鼠切除卵巢后瘦素水平显著下降 (p <0 0 5 ) ,切除卵巢后单独埋植雌激素、孕酮不能阻止瘦素水平的下降 ,但是当埋植孕酮和雌激素复合物时瘦素的水平得到恢复。这些结果说明性腺激素对于瘦素的分泌有重要的调节作用。单独的雌激素或孕激素对瘦素分泌没有影响 ,但雌激素与孕激素复合物却对于瘦素的分泌有重要的调节作用。结论 :妊娠后期高水平的雌激素、孕激素等性腺激素的综合作用可能促成了该期瘦素水平的上升。更多还原

【Abstract】 Objective To investigate the effect of sex hormones on the secretion of leptin and the causative factor of the gestational leptin spike in the golden hamster. Methods Three months old female golden hamster were used as animal model. As a source of high level estradiol and progesterone, silicane rabber tubes impregnates with estradiol and progesterone were prepared and their bioactivity were determined. Antisera against estradiol and progesterone were prepared and activity tested to be used? for the elimination of the effects of endogenous hormones on leptin secretion in the subsequent experiments. Biological activity of the antiserum was determined by evaluating effects of these antisera on the weight of uterus or ovary. Groups of pregnant animals were ovariectomied during day 11 of pregnancy to explore the effect of the gonad on the secretion of leptin. Groups of virgin animals were ovariectomied and the silicone rubber tubes containing estradiol and progesterone were implanted to determine the effect of high level estradiol and progesterone on the secretion of leptin in vivo. Results Plasma concentration of leptin decreased and the gestational leptin profile disappeared with absence of the secrection spike on day 12 after ovarectomy on the day 11 of pregnancy. Injections of antiserum against estradiol or progesterone had no significant effect on the plasma concentration of leptin. Leptin level significantly decreased after ovarectomy in the virgin golden hamsters ( p <0 05). Implantation of silicone rubber tubes of estradiol or progesterone after overectomy could not restore leptin levels, but implantation of tubes containing both estradiol and progesterone could prevent the decrease of leptin levels. Conclusion Our results suggested that sex hormones had important regulatory effect on the secretion of leptin. Estradiol plus progesterone had stimulatory effects on the secretion of leptin in vivo. High estradiol and progesterone levels during pregnancy was at least one of the most important causative factors of the appearance of leptin profile with a secretion spike in the golden hamster.更多还原