【摘要】 目的　在单细胞水平上 ,研究类风湿关节炎 (RA)患者滑液及外周血中单核 /巨噬细胞、T淋巴细胞上趋化因子受体CCR5及CXCR3的表达 ,并测定CCR5的配体MIP 1β、激活正常T细胞表达和分泌因子 (RANTES)及T细胞亚群 ,探讨其在RA发病中的作用机制。方法　分离RA患者滑液单个核细胞 (SFMC)、外周血单个核细胞 (PBMC)及正常人PBMC(对照 ) ,以三色荧光素标记物进行流式细胞术分析CD14 + 、CD3+ 细胞膜表面趋化因子受体CCR5、CXCR3及细胞内趋化因子MIP 1β、RANTES和细胞因子白细胞介素 (IL) 4、干扰素 (IFN) γ的表达率。 结果　与PBMC相比 ,RA患者SFMC中的单核 /巨噬细胞、T细胞上趋化因子受体CCR5及CXCR3表达显著增高 ;且单核 /巨噬细胞分泌趋化因子MIP 1β、RANTES的百分比较高 ;SFMC中Th1细胞亚群 (即IFN γ+ T细胞 )的表达率与滑液及外周血中T淋巴细胞上趋化因子受体CCR5及CXCR3的表达率显著相关。结论　趋化因子受体CCR5 + 、CXCR3+ 的单核 /巨噬细胞、T细胞积聚在RA患者的病变关节内 ,且单核 /巨噬细胞分泌较多趋化因子 ;CCR5、CXCR3可能参与调节细胞移动 ,与Th1细胞亚群及其“伙伴”细胞在RA关节内积聚有关 ;可能与RA的发病相关。更多还原

【Abstract】 Objective To investigate the expression of chemokine receptors CXCR3 and CCR5 on CD14 + and CD3 + cells from patients with rheumatoid arthritis (RA),and to analyse the CCR5 ligands(MIP 1β and RANTES) and T phenotype of T cells.Methods The expression of CXCR3 and CCR5 on CD14 + or CD3 + cells,and the percentage of MIP 1β and RANTES in CD14 + cells in synovial fluid (SF) and peripheral blood (PB) mononuclear cells from RA patients and healthy subjects were studied by three color flow cytometry.And the percentage of IFN γ and IL 4 in CD3 + cells was detected by the same method.Results The percentage of CCR5 +,CXCR3 +,CD14 +or CD3 +cells was much higher in SFMC than in PBMC,and the percentage of MIP 1β + or RANTES +in CD14 +cells form RA patients was also higher in SFMC than in PBMC or in PBMC from healthy subjects.The percentage of CCR5 + or CXCR3 + T cells and the Th1 cells showed consistent.The percentage of CCR5 + or CXCR3 + T cells in SFMC was correlated with patient′s erythrocyte sedimentation rate (ESR) and C reactive protein (CRP).Conclusion A large number of CCR5 + or CXCR3 +CD14 +or CD3 + cells accumulate in the RA patient′s injured articular cavity.Some CD14 + cells secrete MIP 1β or RANTES,which activate and attract the CCR5 +or CXCR3 +cells,causing the accumulation of CCR5 +or CXCR3 +Th1 cells and macrophages.This phenomenon maybe has a close relation with the RA.更多还原