【摘要】 以余甘子叶片为材料,研究了余甘子基因组DNA提取方法及RAPD反应条件.结果表明,采用改良的SDS方法,提取的DNA质量较高,适宜于RAPD-PCR分析.在25μL反应体积中,RAPD分析的优化反应体系为:0.6mmol·L-1Mg2+、500-600μmol·L-1dNTP、300nmol·L-1随机引物、25ngDNA、1.00-1.25UTaqDNA聚合酶.更多还原

【Abstract】 The method of genomic DNA extraction and the optimization of RAPD analytic conditions were studied in Phyllanthus emblica L. The results showed that high grade genomic DNA was obtained by the revised SDS method. The optimal PCR system for RAPD analysis was as follows: 0.6 mmol·L-1 Mg2+, 500-600 μmol·L-1 dNTP, 300 nmol·L-1 random primer, 25 ng DNA template, 1.00-1.25 U Taq polymerase in 25 μL reaction solution.更多还原