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甜叶悬钩子茎尖微繁技术研究
Study on the Micro-Propagating of Stem Tip of Rubus suavissimus S.Lee
【摘要】 对甜叶悬钩子的茎尖微繁技术进行了研究。结果表明:在MS培养基中附加植物生长调节剂BA0 2~0 5mg·L-1和NAA0 2mg·L-1,诱导茎尖效果最佳,诱导率高达76%;在附加植物生长调节剂BA0 6mg·L-1和NAA0 3mg·L-1的MS培养基上进行丛芽继代培养,增殖倍数可达7 5倍;壮苗生根培养用1/2MS培养基附加植物生长调节剂NAA0 7mg·L-1和活性炭为佳。
【Abstract】 The technique of propagating stem tip of Rubus suavissimus S.Lee were studied in this paper.The results showed that:The MS culture medium appending 0.2~0.5mg.L BA and 0.2mg.L NAA were better in micro-propagating of stem tip,The induction rate were 76%;Tufty bud were secondly incubated in the MS culture medium appending 0.6mg·L BA and 0.3mg·L NAA;Its proliferous multiple reached 7.5;The 1/2MS culture medium appending 0.7mg·L NAA and active carbon were better in rooting of vigorous seedlings.
【关键词】 甜叶悬钩子;
茎尖微繁;
外植体;
【Key words】 Rubus suavissimus S.Lee; micro-propagating of stem tip; explant;
【Key words】 Rubus suavissimus S.Lee; micro-propagating of stem tip; explant;
【基金】 福建省林业厅《甜菜繁育、栽培与加工技术及其应用的研究》(98-6-4)的部分研究内容
- 【文献出处】 福建林业科技 ,Journal of Fujian Forestry Science and Technology , 编辑部邮箱 ,2003年04期
- 【分类号】S723.1
- 【被引频次】9
- 【下载频次】83