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美洲大蠊血细胞对金龟子绿僵菌CQMa102菌株的免疫反应

Cellular Defence Reaction of American Cockroach , Periplaneta americana to Metarhizium anisopliae Isolate CQMa102

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【作者】 钟伟殷幼平

【Author】 ZHONG Wei 1, YIN You ping 2 (1, Department of Plant Protection, Southwest Agricutural University, Chongqing 400716,China; 2,Genetic Engineering Research Centre, Chongqing Uiversity, Chongqing 400030,China)

【机构】 西南农业大学植物保护学院重庆大学基因工程研究中心 重庆400716重庆400030

【摘要】 将蜚蠊的非致病菌———金龟子绿僵菌 (Metarhiziumanisopliae)CQMa1 0 2菌株的分生孢子注入美洲大蠊 (Periplanetaamericana)血腔后 ,诱导了蜚蠊血淋巴强烈的免疫反应 ,其血细胞数量发生了显著变化 ,血细胞通过吞噬作用及形成结节使绿僵菌孢子钝化 ,萌发受到抑制 ,或被彻底分解 ;注射高浓度孢子时 ,蜚蠊的免疫体系受到严重破坏 ,容易感染细菌而死亡 ;但注射低浓度孢子却能够增强蜚蠊的免疫机能。生理盐液也能诱导蜚蠊血淋巴的免疫反应。

【Abstract】 Injection of conidia solution of Metarhizium anisopliae isolate CQMa102 into the hemocoel of Periplaneta americana caused significant changes of the total hemocytes of the cockroach. P. americana eliminated large amount of the spores efficiently by phagocytosis and nodulation.The experiment shows that the immune system of the cockroach was hardly injured after injected with high dose spores, and the higher dose of spores was injected,the higher mortality of the insect was. However, low dose injection strengthened the immune ability of the insect in certain degree. The saline slightly induced the immune reaction of the insect.

  • 【文献出处】 重庆大学学报(自然科学版) ,Journal of Chongqing University(Natural Science Edition) , 编辑部邮箱 ,2003年06期
  • 【分类号】R184
  • 【被引频次】13
  • 【下载频次】143
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