【摘要】 运用SDS 聚丙烯酰胺凝胶电泳的方法,以马心肌红蛋白为代表研究蛋白质氨基生物素化的条件,包括蛋白质与生物素量的关系、反应物浓度、反应完全所需的时间以及蛋白质助溶剂对反应的影响等,为基于生物素 抗生物素蛋白质相互作用的亲和分离,简化复杂蛋白质组体系,实现蛋白质的分析鉴定奠定基础.更多还原

【Abstract】 Using SDS-PAGE technique, some conditions for protein biotinylation including quantity relationship between protein and biotinylated reagent, reactant concentration, reaction time, and effect of solvent which prompt protein dissolvation, etc., have been experimentally investigated. The results provide solid foundation for further study on affinity isolation and simplification of the complex proteome system, and for the advanced identification of proteins.更多还原