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水稻(OryzasativaL.)磷酸盐转运蛋白编码基因的表达研究(英文)

Differential Expression of PTW-J, a Possible Gene Encoding High Affinity Phosphate Transporter in Rice (Oryza sativa L.)

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【作者】 明凤郭滨梁斌王敬文韩颖颖沈大棱

【Author】 MING Feng, GUO Bin, LIANG Bin, WANG Jingwen, HAN Yingying, SHEN Dalingering, Research Centre of Gene Diversity and Designed Science and Ecological Engineering,School of Life Sciences, Fudan University, Shanghai 200433, China)

【机构】 复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室基因多样性与设计农业中心生物多样性与生态工程教育部重点实验室复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室基因多样性与设计农业中心生物多样性与生态工程教育部重点实验室 上海 200433上海 200433上海 200433

【摘要】 研究揭示了水稻磷酸盐转运蛋白编码基因的表达.低磷处理下,根系与叶片中的PTW J转录子迅速表达,处理7d时达到最高表达量.PTW JmRNA的诱导积累具有元素的专一性,因为在缺氮、缺钾与缺铁情况下此基因不表达.缺磷处理后恢复供磷,PTW J转录子积累量明显降低.这些结果说明,PTW J基因表达是对磷素缺乏的早期反应机制.

【Abstract】 High affinity phosphate transporter plays an important role in plant adapting to low phosphorus (Pi) stress. In this study, the expression of the PTWJ was characterized: the PTWJ transcripts were highly induced in roots and leaves during Pi starvation, a significant increase in the PTWJ mRNA was observed after 7 d of Pi starvation. Induction of the PTWJ mRNA appeared to be a response specific to Pi starvation. The amount of PTWJ transcript decreased rapidly when Pistarved rice plants were resupplied with Pi. The results suggested that PTWJ gene expression may be a part of the early Pi starvation response mechanism in plants.

【基金】 SupportedbyChinaPost DoctoralFoundation (2 0031);SupportedbyFudanUniversityGeneticssub jectsupportingitemfound
  • 【文献出处】 复旦学报(自然科学版) ,Journal of Fudan University , 编辑部邮箱 ,2003年04期
  • 【分类号】S511
  • 【被引频次】6
  • 【下载频次】138
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