【摘要】 目的　研究FLIP在非小细胞肺癌 (NSCLC)中的表达并观察其与细胞凋亡的关系。方法　采用免疫组化技术检测FLIP在 48例NSCLC和 16例肺良性疾病组织中的表达 ,并应用TUNEL技术检测 48例NSCLC的凋亡情况。结果　肺癌组织中FLIP的表达率为 83 .3 3 % ( 4 0 /4 8) ,明显高于肺良性病变组织( 2 5 .0 0 % ) (P <0 .0 1)。FLIP的表达水平与TNM分期及淋巴结转移有密切关系 ,但与肿瘤组织学分型、分化程度无明显关系。FLIP在肺癌组织中的表达水平与癌细胞凋亡指数 (AI)未见明显相关性 (r =-0 .2 11,P >0 .0 5 )。结论　FLIP的过量表达在NSCLC的发生发展中起重要作用 ,但与细胞凋亡无明显关系。更多还原

【Abstract】 Objective To investigate the relation between FLIP expression and apoptosis in non small cell lung cancer (NSCLC). Methods FLIP expression was examined in forty eight paraffin embeded NSCLC samples and 16 benign pulmonary disease tissues by immunohistochemistry method. Apoptosis of NSCLC cells was detected by TUNEL technique. Results The positive rate of FLIP expression in NSCLC was 83.33%(40/48), which was significantly higher than that in benign pulmonary disease tissues (P<0.01). The expression level of FLIP was closely related to TNM stages and lymph node involvement, but not to histological classification and cell differentiation. No correlation was observed between the expression of FLIP and apoptosis index of tumor cells (r=-0.211,P> 0.05 ). Conclusion Overexpression of FLIP may be involved in the progression of NSCLC, but its expression may not be related to cell apoptosis in NSCLC.更多还原