【摘要】 从天津地区各区县养鸡场分离 ,鉴定出 45株鸡致病性大肠杆菌。对 45株致病性大肠杆菌进行血清型鉴定 ,共定型出 3 1株 ,分属 1 4个血清型 ,其中 O78、O88、O2 、O4 5、O53、O14 5为优势血清型 ,占定型菌株的 58.3 %。提取 O78、O88、O2 、O4 5、O53和O14 56个血清型 1 8个分离株的外膜蛋白 ( Outermembrane protein,OMP) ,测定外膜蛋白型 ( Outmembrane protein patterns,OMPS) ,SDS-PAGE分析表明这些分离株共产生了 3个 OMP型 ( 1～ 3型 )。 OMP-1型为 O78、O88、O2 、O53、O14 55个血清型分离株所共有 ,OMP-2型为 O78、O4 5、O14 53个血清型分离株所共有。这表明同一血清的菌株可能属于完全不同的 OMP型 ,而血清型不同的菌株也可能为同一 OMP型。更多还原

【Abstract】 45 strains of Pathogenic Escherichia coli(E.coli) were isolated from chickens of fowl farms in Tianjin regions and were studied for O serotypes. 31 of them were identified as O type belonged to 14 serogroups and the O78,O88,O2,O45,O53and O145were Predominant.Outer membrane proteins (OMP) were prepared from 18strains belonged to O78,O88,O2,O45,O53 and O145 serogroups. SDSPAGE analysis showed that 3 OMP patterns (1~3 patterns) were found from these strains. OMP1 pattern was found from the O78,O88,O2,O53 and O145 serogroups,OMP2 pattern was found from the O78,O88 and O2 serogroups, OMP3 pattern was isolated from O78,O45, and O145 serogroups. These results showed that strains of different O serogroups of chicken E.coli could have the same OMP pattern and the strains belonged to the same O serogroup could have different OMP patterns.更多还原