【摘要】 目的探讨高温下新生大鼠海马锥体神经元凋亡形态学及生物化学变化。方法建立体外海马神经细胞培养模型,以37、40、42 ℃3种温度作为环境刺激因素,应用流式细胞仪、荧光染色、琼脂糖凝胶电泳和原位细胞凋亡检测试剂盒对其观察。结果随作用温度升高细胞凋亡率及凋亡小体数增加,电泳呈现DNA Ladder表现,荧光显微镜观察能够看到凋亡小体及其出芽现象。结论高温能够诱导体外培养的大鼠海马锥体细胞发生凋亡,且不同温度诱导其凋亡的程度及时程表现亦不同。更多还原

【Abstract】 Objective To investigate the changes in the morphology and biochemistry of neonatal rat hippocampal pyramidal cells (HPCs)apoptosis in high-temperature environment. Methods In vitro culture of HPCs was established and then subject-ed to environmental stimulation with high temperatures (37, 40, and 42 ℃respectively) for 1 h. All the cells were collected at different time points after the treatment and examined by flow cytometery, fluorescence staining, agarose gel electrophoresis and in situ cell apoptosis detection kit. Results The apoptotic rate and the number of apoptotic bodies increased as the tem-perature was elevated. DNA ladder was presented on agarose gel electrophoresis, and the germination of the apoptotic body was observed by fluorescence microscope. Conclusions Hyperthermia may induce the apoptosis in HPCs in vitro, and the ex-tend of apoptosis and apoptotic behavior may vary with the temperature and treatment duration.更多还原