【摘要】 目的 :研究他莫西芬 (tamoxifen ,TAM)对宫颈癌HeLa细胞增殖的影响 .方法 :应用生长曲线测定、MTT、流式细胞术和激光扫描共聚焦显微镜等技术 .结果 :TAM(1× 10 7mol·L 1)使HeLa细胞的生长曲线向上移动 ;TAM(1× 10 8～ 1×10 6 mol·L 1)可显著促进HeLa细胞增殖 ;TAM(1× 10 6 mol·L 1)促进细胞G1/S期转化 ,并显著促进细胞内Ca2 +释放 .结论 :TAM可能对子宫颈癌细胞的生长和分化具有重要的调节作用 ,这种调节作用可能是通过影响细胞周期和胞内Ca2 +水平而实现的 .更多还原

【Abstract】 AIM: To investigate whether tamoxifen is involved in the proliferation of cultured HeLa cell line, which is derived from cervical carcinoma cell line of human beings. METHODS: The level of cell proliferation was assessed by drawing the proliferation curves of HeLa cells, detecting OD value of MTT assay and using flow cytometry and laser scanning confocal microscope. RESULTS: Tamoxifen (1×10 -7 mol·L -1 ) shifted the curve upward. Tamoxifen (1×10 -8 ～1×10 -6 mol·L -1 ) significantly stimulated the cell proliferation and facilitated (1×10 -6 mol·L -1 ) G 1/S transfer of the cell cycle as well as greatly increased the releasing of Ca 2+ in the cells. CONCLUSION: Tamoxifen may modulate the proliferation and the differentiation of cultured HeLa cervical carcinoma cells by affecting the cell cycle and Ca 2+ in the cells.更多还原