【摘要】 运用UV-3101PC型紫外-可见-红外分光光度计及皮秒时间分辨荧光光谱技术对20℃、42℃、48℃3个温度下,PS 核心复合物的吸收光谱和时间分辨荧光光谱的变化进行分析,结果发现: 1 在PS 核心复合物中至少存在以下几种具有特征吸收峰的chla分子,CP43:chla660/661 chlaa:a代表吸收峰 、chla669、chla671、chla682/683;CP47:chla660/661、chla669、chla671/672、chla688、chla680;RC:chla680、chla670、chla684、chla673/674、chla682/683、chla660.吸收峰波长随测量温度等条件的不同而略有变化. 2 荧光发射谱组分的峰值随温度的升高而发生明显的蓝移,这是由于热诱导改变了蛋白质的结构,从而使生色团间的距离和 或 方位受到了影响,而chla分子的结构并未发生变化,因此导致chla分子间激子相互作用被破坏,从而产生了发射峰蓝移的现象. 3 20℃、42℃时核心天线向反应中心的能量传递是高效有序的. 4 反应中心中与蛋白质存在不同结合的chla分子,以及核心天线中吸收不同波段光的chla分子与蛋白质结合方式随温度变化存在不同反应.更多还原

【Abstract】 Temperature is one of the most important factors,which can influence the progress of photosynthesis.In the range of suitable temperatures,photosynthesis can carry out normally,above this temperature,it will be destroyed.In this essay,we dealed PSⅡ core complex isolated from spinach with three different temperatures 20℃,42℃,48℃,analyzed with absorption spectroscopy and time-resolved fluorescence spectrum measurement.The results were as follows:(1) There are several chla in the core complex of PSⅡ:CP43:chla660/661(chlaa:a represents absorption peak),chla669,chla671,chla682/683;CP47:chla660/661,chla669,chla671/672,chla688,chla680;RC:chla680,chla670,chla684,chla673/674,chla682/683,chla660.(2) Almost all of peaks of components in fluorescence spectrum have blue shifts with increasing temperatures,due to thermal effect.Temperature has changed the microenvironment of chromospheres,such as position,orientation and distance between the chromospheres.(3) Core antenna complex transfer energy to RC efficiently at 20℃,42℃.(4) Chla in RC and chla absorbing different wavelength in core antenna complex connected with proteins at different positions have different responses with changing temperature.更多还原