【摘要】 从甘农薯1号马铃薯试管苗叶片中提取总DNA,根据Genebank报道的马铃薯多酚氧化酶基因 POT32 序列,设计合成了带有BamH 、Sac 特定酶切位点的2对特异引物,通过PCR获得1840bP和476bP的扩增产物.测序结果表明,1840bp的扩增产物与Genebank中发表的序列一致,476bp的扩增产物正是欲扩增的马铃薯多酚氧化酶基因的同源片段.将2个扩增产物反向连接到表达载体PC3中,通过双酶切鉴定后,按直接导入法实现反义基因表达载体质粒向农杆菌EHA105的导入;并经PCR鉴定证明,此质粒已整合到农杆菌Ti上.更多还原

【Abstract】 In present studies,the general DNA was extracted from the potato leaves of "Gannong No.1".Two pairs of special primer were designed according to POT32 sequence by Genebank published,and two amplification products were obtained (one is 1 840 bp,another is 476 bp).Antidirect linked them to the PC3 expression vector,and checked them with two enzymes cutting.Then made the antisense expression vector into Agrobacterium EHA105 by the method of directing translation.The conclusion was that vector have been into EHA105.更多还原