【摘要】 以小麦为实验材料,研究了盐胁迫对根质膜H⁺-ATPase、Ca²⁺-ATPase活性及H⁺-ATPase蛋白表达的影响。结果显示:50、100、150mmol/L的NaCl处理72h后,小麦根质膜H⁺-ATPase、Ca²⁺-ATPase活性均降低。100mol/L NaCl对质膜ATPases活性的抑制程度随处理时间的延长而增强,在处理24h后,H⁺-ATPase和Ca²⁺-ATPase的活性分别降为对照的72％和75％,而处理72h后,酶活性分别减小到对照的50％和48％。50、100、150mmol/L的NaCl直接作用于提取的质膜微囊,H⁺-ATPase的活性分别降低约5％、8％和16％。Western blotting分析结果显示100mmol/L NaCl处理72h后,质膜H⁺-ATPase的含量与对照比有所减少。本研究表明:盐胁迫抑制小麦根质膜H⁺-ATPase、Ca²⁺-ATPase的活性,酶含量的减少可能是盐胁迫导致质膜H⁺-ATPase活性降低的原因。更多还原

【Abstract】 The effects of salinity on the activities of the plasma membrane H+-ATPase、Ca2+-ATPase and the expression of the H+-ATPase were investigated when wheat roots were subjected to NaCl stress. The activities of plasma membrane H+-ATPase and Ca2+-ATPase were greatly inhibited by NaCl 50、100、150 mmol/L for 72 h. The H+-ATPase and Ca2+-ATPase activities were decreased to 72% and 75% after treatment in the presence of 100 mmol/L NaCl for 24 h,and to 50% and 48% after treatment for 72 h,respec-tively. When the plasma membrane vesicles were treated with 50、100 and 150 mmol/L NaCl directly,the H+-ATPase activities were suppressed by 5%, 8% and 16%, respectively. Western blotting analysis showed that the amount of the H+-ATPase was reduced after 72 h under 100 mmol/L NaCl treatment. Our results indicated that NaCl treatment induced inhibition in the plasma membrane H+-ATPase、Ca2+-AT-Pase activity,and that the decrease in the contents of plasma membrane H+-ATPase might account for the inhibitory effect of NaCl on the the H+-ATPase activity.更多还原