【摘要】 漆酶可催化酚类化合物和芳香胺的氧化,在小分子介体物质存在下,漆酶氧化范围可进一步扩大。白腐菌Pleurotusostreatus3.42产漆酶能力强,可成为工业用漆酶的重要生产者。研究结果表明,其在静止培养条件下,菌体生长及产漆酶能力都优于摇床培养,同时限氮培养也利于漆酶的生产。在所选的碳氮源中,纤维素和酪蛋白为最佳碳氮源。在诱导剂中ABTS诱导效果最佳,添加后漆酶酶活可达1000U／mL。更多还原

【Abstract】 Laccase is capable of catalyzing the oxidation of a broad number of phenolic compounds and aromatic amines. The oxidized substrates by laccase can be extended in the presence of small molecular mediators. Pleurotus ostreatus 3.42 is an excellent producer of the industrially important enzyme laccase. Growth and laccase production in static cultivation are superior to that in agitated cultivation, and Nlimited culture is of benefit to laccase production. By using cellulose and peptone as carbon and nitrogen source, a higher activity level is obtained. Experiment demonstrates that ABTS (1?mmol) is the best inducer of laccase production, reaching a maximum value of 1?000?U/mL, approximately.更多还原