节点文献

应用分子信标荧光PCR定量检测乙型肝炎病毒核酸的评价

Evaluation of a Molecular Beacon Fluorescence PCR Kit for Quantitative Detection of Hepatitis B Virus Nucleic Acid

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 蔡枫金根娣顾士民蒋伟伦

【Author】 Cai Feng, Jin Gen - di , Gu Shi - min, Jiang Wei - lun(Shanghai Municipal Infectious Disease Hospital, Shanghai 200083)(Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025)

【机构】 上海市传染病医院上海第二医科大学附属瑞金医院上海市传染病医院 上海 240083上海 200025上海 240083上海 240083

【摘要】 目的:分子信标(Molecular Beacon)是近年新发展的一种荧光标记发夹形寡核苷酸探针。本文目的在于对国产分子信标荧光PCR定量检测HBV核酸试剂盒(厦门泰伦生物工程有限公司的“乙型肝炎病毒荧光PCR定量检测试剂盒”)的主要性能进行评价。方法:以Roche公司的乙型肝炎病毒(HBV)定量检测试剂盒AMPLICOR HBV MONITORTM Test Kit为参比,对分子信标定量检测临床血清标本HBV DNA的结果,进行比较和分析。结果:根据两种试剂盒定量检测34份乙型肝炎和非肝炎血清及一份系列10倍稀释血清(含高水平HBV DNA)的数据,并以Roche试剂盒的结果为参比标准,分子信标试剂盒定量检测HBV DNA的相对敏感性、特异性和符合率均为100%(分别为:19/19,15/15和34/34);其定量检测HBV DNA的线性范围和下限分别为103~108 copies/ml和103 copies/ml;两种试剂盒定量检测HBV DNA的相关系数(r)=0.987。结论:初步结果表明:该国产分子信标荧光PCR定量检测HBV核酸试剂盒的主要性能,与Roche公司的AM-PLICOR HBV MONITORTM Test试剂盒相似,并具有较后者更宽的线性定量范围。

【Abstract】 Objective: To evaluate a molecular beacon fluorescence PCR kit for quantitative detection of hepatitis B virus nucle-ic acid (produced by Xiamen Tailun Biothec Co, Fujian, China) .Methods: This molecular beacon kit was compared with Roche"AM-PIJCOR HBV MONITOR?Test" kit for quantitative detection of HBV DNA in clinical serum specimens.Results: Based on the data that the HBV DNA levels in 34 sera from patients with and without hepatitis B and a serial 10 fold diluted serum (containing HBV DNA) detected by the two kits and using the results by the Roche kit as reference Standard, the relative specificity, sensitivity and over-all accordance of the molecular beacon kit were all 100% (19/19, 15/15, and 34/34, respectively); the linear quantitative range and real detection lower limit of this kit were itf ~ itf and itf HBV DNA copies/rol, respectively; the correlation coefficient (r) of HBV DNA quantitative detection between the two kits was 0.987.Conclusion: Preliminary results indicate that the main efficacy of the molecular beacon fluorescence PCR kit for guantitative detection of hepatitis B virus nucleic acid is similar to the Roche" AM-PLICOR HBV MONTTOR?Test" kit for the same purpose and the former kit has a wider h’near quantitative range.

  • 【文献出处】 中国医药导刊 ,Chinese Journal of Medicinal Guide , 编辑部邮箱 ,2003年01期
  • 【分类号】R446.5
  • 【下载频次】108
节点文献中: 

本文链接的文献网络图示:

本文的引文网络