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钾通道阻断剂和一氧化氮对肺动脉平滑肌细胞钾电流的作用

Effect of potassium channel inhibitor and nitric oxide on potassium current of pulmonary artery smooth muscle cells

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【作者】 姚小鹏钱卫珠李强朱海峰黄怡白冲董宇超刘忠令

【Author】 YAO Xiao Peng 1, QIAN Wei Zhu 2, LI Qiang 1, ZHU Hai Feng 3, HUANG Yi 1, BAI Chong 1, DONG Yu Chao 1, LIU Zhong Ling 1 (1. Department of Respiratory, Changhai Hospital, Second Military Medical University, Shanghai 200433,China; 2. International Joint Cancer Institute, Second Military Medical University;3. Hypoxic Cardiovascular Lab, Shanghai Life Sciences Research Institute,Chinese Academy of Science)

【机构】 第二军医大学长海医院呼吸内科第二军医大学国际肿瘤研究所中国科学院上海生命科学研究所低氧心血管实验室第二军医大学长海医院呼吸内科 上海200433上海200433上海200433

【摘要】 目的 :研究钾通道阻断剂、一氧化氮 (NO)对继代培养大鼠肺动脉平滑肌细胞 (PASMC)钾电流 (IK)的作用。 方法 :使用全细胞膜片钳技术观察继代培养大鼠 PASMC IK 及钾通道阻断剂格列本脲 (Gli) ,四乙胺 (TEA) ,4 -氨基吡啶 (4- AP)和 NO供体 S-亚硝基乙酰青霉胺 (SNAP)对其的作用。 结果 :继代培养大鼠 PASMC较急性分离者之 IK 明显降低。 4 - AP和高浓度TEA显著降低继代培养大鼠 PASMC之 IK,SNAP显著增加继代培养大鼠 PASMC之 IK。结论 :继代培养大鼠 PASMC钾通道特性发生改变 ,但通道阻断剂和 NO仍能显著改变其活性。

【Abstract】 Objective: To study the effect of potassium channel inhibitor and nitric oxide(NO) on the potassium current of sub cultured rat pulmonary artery smooth muscle cells(PASMC). Methods: The potassium current (I K) of sub cultured rat PASMC and the effect of potassium channel inhibitor Gli, TEA, 4 AP, and NO donor SNAP on it were observed by whole cell patch clamp techniques. Results: The I K of sub cultured rat PASMC was remarkably reduced than that of the acute dispersed ones. 4 AP and high concentration TEA remarkably reduced I K of sub cultured rat PASMC, while SNAP remarkably increased the I K. Conclusion: The character of sub cultured rat PASMC potassium channel is remarkably changed, but the potassium channel inhibitors and NO can still change its activity remarkably.

  • 【文献出处】 第二军医大学学报 ,Academic Journal of Second Military Medical University , 编辑部邮箱 ,2003年02期
  • 【分类号】R332
  • 【被引频次】6
  • 【下载频次】86
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