【摘要】 目的　研究转化生长因子 (transforminggrowthfactorTGF -β1)对大鼠同种胰岛移植物体内外的内分泌功能及存活的影响。方法　构建TGF -β1的真核表达载体pcDNA3 -TGF ,并将其转染纯化后的Wistar大鼠胰岛 ,RT -PCR检测TGF -β1和大鼠胰岛素 (Insulin 1Ins1)在实验组和对照组胰岛中的表达 ,放射免疫法 (radioimmunologicalassayRIA)检测体外培养液中的胰岛素含量 ;链脲霉素 (streptozotocinSTZ) 60mg/kg腹腔内注射制作SD大鼠模型 ,分别将实验组和空白对照组Wistar胰岛移植到受体SD大鼠的肾包囊下 ,观察其在体内逆转糖尿病的作用以及作用时间的长短 ,并检测受体大鼠的糖耐量。结果　实验组胰岛有TGF -β1和Ins1的表达 ,而对照组仅有Ins1的表达 ;实验组体外培养液中的胰岛素含量在基础相和刺激相分别为 ( 3 86± 1 2 4)ng/ml和 ( 8 43± 1 5 9)ng/ml ,对照组体外培养液中的胰岛素含量在基础相和刺激相分别为 ( 4 13± 1 45 )ng/ml和 ( 8 95± 1 74)ng/ml,组间无显著性差异 (P >0 0 5 ) ,而组内有显著性差异 (P <0 0 5 ) ;实验组平均正常血糖维持时间 ( 2 6 8± 2 9d)比对照组 ( 13 2± 4 5d)显著延长。对照组糖耐量曲线均明显高于实验组。结论　TGF -β1对胰岛移植物的内分泌功能无损害作用 ,但是可以更多还原

【Abstract】 Objective To investigate the effect of transforming growth factor β1 on secretion function of rat islet allograft in vivo and in vitro.Methods Expressing vector of rat TGF-β1 was constructed and transfected to purify Wistar rat islets. Expression of TGF-β1 and rat insulin1 gene (Ins1) in experiment and control group was detected by RT-PCR; Streptozotocin 60mg/kg intra-abdominal injection prepared the SD rat diabetic model. Insulin of cultured islets at basal and stimulated phase in vitro was detected by RIA; Islet allotransplantation was performed under recipient SD rats’ renal subcapsule. The level of blood glucose was recorded before and after transplantation. The glucose tolerance was also tested.Results Expression of both TGF-β1 and Ins1 were detected in experimental group, but only Ins1 was detected in control one; Experiment and control islets secreted 3 86±1 24ng/mL and 4 13±1 45ng/mL at basal phase, 8 43±1 59ng/mL and 8 95±1 74ng/mL at stimulating phase, respectively.No significant difference was observed between two groups, but it was in groups. Experimental group’ mean normal glycemia value (26 8±2 9) days was significantly longer that control group (13 2±4 5 days). Control group’s glucose tolerance curve was significantly above experiment one’s.Conclusion TGF-β1 can prolong the survival of islet allograft while it does not impair graft’s endocrine function.更多还原