【摘要】 目的　通过观察AT1受体的特异性拮抗剂氯沙坦 (Losartan)对兔腹主动脉球囊成形术后内膜增殖和巨噬细胞表达的影响 ,探讨氯沙坦治疗血管再狭窄的机制。方法　采用内皮剥脱加高脂饮食 (含 1 5 %胆固醇 )喂养 8周制作兔腹主动脉粥样硬化膜型 ,然后对狭窄部位行球囊成形术。术后氯沙坦组给予氯沙坦 10mg·kg-1·day-1口服 ,对照组喂生理盐水。 4周后取腹主动脉行组织形态学观察 ,用免疫组织化学方法分析巨噬细胞。结果　对照组内膜厚度 /中膜厚度比值为 0 65± 0 17,氯沙坦组则减少为0 44± 0 11(P <0 0 1) ,对照组内膜面积 /中膜面积比值为 0 74± 0 16,氯沙坦组则减少为 0 5 4± 0 13(P <0 0 1)。氯沙坦组新生内膜中巨噬细胞数亦较对照组显著减少 (P <0 0 1)。结论　氯沙坦通过抑制AngⅡ的作用进而抑制巨噬细胞的浸润 ,抑制炎症反应 ,从而减轻再狭窄更多还原

【Abstract】 Objective Objective To investigate the effects of losartan on neointimal proliferation and macrophage count in neointima.Methods The rabbits underwent abdominal aorta balloon de-endothelialization,then received a 1 5 % cholesterol diet for 8 weeks.Abdominal aorta atherosclerosis model was performed in 16 rabbits.Immediately after operation, losartan was orally administered to the rabbits of the losartan group (10mg·kg -1 ·day -1 ), while the rabbits of the control group were given normal saline. Four weeks later the animals were killed and the excised artery segments were prepared for histomorphological observation and macrophages were investigated by immunohistochemistry analysis.Results Compared with the control group, the IT/MT ratio and IA/MA ratio of the losartan group were significantly reduced(P<0 01). Also, macrophages in the neoinima were significantly diminished(P<0 01).Conclusions Losartan could significantly reduce neointimal proliferation after balloon angioplasty may be through inhibiting angiotensinⅡ,and thus inhibiting infiltration of macrophages into neointima.So inflammatory reaction is suppressed and restenosis is relieved.更多还原