【摘要】 目的 探讨哮喘儿童血IgE和T细胞亚群、细胞因子的动态变化及临床意义。方法 应用免疫萤光法及双抗夹心酶联免疫吸附试验（ELISA）分析方法对45例哮喘儿童发作期和缓解期分别测定IgE,T细胞亚群和细胞因子。对照组为20例健康儿童。结果 哮喘发作期、缓解期CD₃⁺,CD₄⁺ T细胞及CD₄⁺/CD₈⁺高于对照组,差异有显著性（P<0.05或0.01）,CD₈⁺ T细胞与对照组比差异无显著性（P>0.05）。发作期CD₄⁺ T细胞及CD₄⁺/CD₈⁺高于缓解期（P<0.05）。发作期IL-2,EFN-γ低于对照组（P<0.01或0.05）,IL-4,IL-6,IL-8和IgE高于对照组（P<0.01或0.05）;缓解期IL-2,IFN-γ低于对照组（P<0.01）,IL-4,IL-8,IgE高于对照组（P<0.05或0.01）,缓解期IL-6与对照组比较差异无显著性（P>0.05）。结论 儿童哮喘在发作期和缓解期均存在着免疫功能紊乱,提示儿童哮喘应长期抗变应性炎症治疗。 [中国当代儿科杂志,2003,5（1）:23-26]更多还原

【Abstract】 Objective To study the changes of serum levels of IgE, T-cell subgroups and cytokines in children with bronchial asthma during the stage of attack and convalescence. Methods The levels of T-cell subgroups were determined by the mono-clone antibody indirect-immuofluorence method and the levels of IgE, IL-2, IL-4, IL-6, IL-8 and IFN-γ were detected using ELISA in 45 children with asthma in the stage of attack and convalescence. Twenty healthy children served as the controls. Results Compared with the controls, there were higher levels of CD3+ and CD4+/ T cells and the ratio of CD4+/CD8+ in the 45 children with asthma in the stage of attack and convalescence ( P<0.05,P<0.01, P < 0.01, respectively), whereas there was no difference in the CD8+ T cell level. The CD4+ T cell level and the ratio of CD4+ /CD8+ of patients were higher in the stage of attack than those in the stage of convalescence ( P <0. 05). The levels of IL-2 and IFN-γ in the asthmatic children in the stage of attack were lower than those of the controls ( P <0.01 or 0.05) and the levels of IL-4, IL-6, IL-8 and IgE were higher than those of the controls ( P <0.01 or 0.05). In the stage of convalescence, the levels of IL-2 and IFN-γ were lower and levels of IL-4, IL-8 and IgE were higher than those of the controls ( P < 0.05 or 0.01), while no difference was noted in the IL-6 level. Conclusions Children with bronchial asthma in the stage of both attack and convalescence have an imrnunologic imbalance. A longer-time anti-alergic treatment administrated for childhood asthma is necessary. [Chin J Contemp Pediatr, 2003, 5(1): 23-26]更多还原