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EDTA萃取法分离自然水体中生物膜胞外聚合物
Extraction of extracellular polymers from biofilms in natural water by EDTA solution
【摘要】 用EDTA水溶液作为萃取剂,对自然水体中培养的生物膜胞外聚合物进行了萃取和分离.通过对糖类、蛋白质、DNA含量的测定,选择了最佳萃取分离条件.结果表明该方法萃取效率高、操作简便、重现性好.
【Abstract】 EDTA solution is applied to extract extracellular polymers from biofilm cultivated in natural water.The optimum extraction conditions are selected by comparing the concentrations of carbohydrates,proteins and DNA.A good extraction efficiency and repeatability have been achieved by the proposed method.Also,the method is easy to operati.
【基金】 国家自然科学基金资助项目(20077011);吉林大学青年教师基金资助项目(2000A24).
- 【文献出处】 东北师大学报(自然科学版) ,Journal of Northeast Normal University (Natural Science Edition) , 编辑部邮箱 ,2003年02期
- 【分类号】X132
- 【被引频次】47
- 【下载频次】624