【摘要】 目的　探讨小鼠烧伤后早期巨噬细胞糖皮质激素受体 (GR)、核转录因子 (NF kB)、肿瘤坏死因子 α(TNF α)和白介素 1β(IL 1β)蛋白表达的变化。 方法　将培养的巨噬细胞株ANA 1注入小鼠腹腔内 ,小鼠行 15 %TBSAⅢ度烧伤 ,收集腹腔巨噬细胞 ,应用免疫组化和Westernblot方法 ,观察烧伤后 2、6小时巨噬细胞GR、NF kB、TNF α和IL 1β蛋白表达的变化。 结果　烧伤后巨噬细胞免疫组化染色GR、NF kB阳性表达减弱 ,TNF α和IL 1β阳性反应增强。Westernblot分析表明烧伤后GR、NF kB蛋白含量降低。 结论　烧伤后巨噬细胞GR、NF kB蛋白减少 ,TNF α和IL 1β增加 ,说明巨噬细胞激活在烧伤后炎症反应中发挥重要作用更多还原

【Abstract】 Objective To detect the early changes of protein expression of GR?NF kB?TNF α?IL 1β of macrophages in mice after burn.?Methods ANA 1 macrophages were collected and injected to the peritoneal cavity of mice,then the mice were inflicted with 15%TBSAⅢ degree burn. The peritoneal macrophages were harvested for the detection of their expression of GR?NF kB?TNF α?IL 1β with immunohistochemical and western blot analysis.?Results After burn injury,the expression of GR and NF kB were reduced,TNF α and IL 1β were increased as shown by immunohistochemical analysis and the contents of GR and NF kB were reduced as found by western blot analysis.?Conclusion The changes of expression of GR,NF kB,TNF αand IL 1β suggest that the macrophages might play an important role in the development of postburn inflammation.更多还原