【摘要】 以邻苯二酚为底物,分析了大久保桃多酚氧化酶(PPO)的最适pH、最适作用温度、酶促动力学参数Km值及其抑制剂。结果表明,大久保桃多酚氧化酶的最适pH值为6.0,最适作用温度34℃,Km=9.7×10-2mmol/L;偏亚硫酸钠、抗坏血酸、谷胱甘肽、巯基乙醇对大久保桃PPO的活性有较强的抑制作用。更多还原

【Abstract】 The optimum pH, optimum temperature, Km and the inhibitors of polyphenol oxidase from Jiubao peaches were analyzed with the catechol substrate. The results showed that optimum pH is 6.0, the optimum temperature is 34℃, the value of Km is 9.7×10-2 mmol/L. Sodium metabisulphide, ascorbic acid, glutathion and mercaptoethnanol were strong inhibitors to PPO. 更多还原