【摘要】 从成都污水处理厂的活性污泥中 ,分离到一株能以邻硝基苯酚 ( o- Nitrophenol,OPN)为唯一碳、氮源生长的细菌。该菌株菌落呈乳白色 ,边缘光滑 ;光学显微镜和扫描电镜观察表明 ,菌体呈短杆状 ,无鞭毛 ,大小约 0 .5× 1.0 μm;革兰氏染色阳性 ;接触酶阴性 ,能利用棉子糖等多种糖类。根据菌体形态、生理生化特征将其鉴定为乳杆菌属的短乳杆菌( L actobacillusbrevis)。用详细研究过的硝基酚降解菌株 Rhodococcus erythropolis H L PM- 1作阳性对照的降解实验表明 ,L.brevis菌株对邻硝基酚有较高的利用率 ,在合适的温度和 p H 条件下 ,18h对邻硝基酚的降解率可达 86.7。用UV-可见光谱和质谱仪对 L.brevis菌株降解邻硝基酚的中间产物进行了分析 ,并对该菌株利用邻硝基酚的可能代谢过程进行了初步探讨更多还原

【Abstract】 A strain of Lactobacillus brevis that can grow with o-Nitrophenol as the sole source of carbon and nitrogen was isolated from the activated sludge in the Chengdu waste water treatment plant.The clolr of the strains clone is creamy,the edge is smooth.The shape of the single cells is pole,about 0.5×1.0μm,without flagellum when inspected by light microscope and Electron Microscope.The cells of the strain were Gram-positive.The hydrogen peroxide reaction of the strain is negative.The strain can use raffinose etc.According to the shape,physiological and biochemical character,it is identified as Lactobacillus brevis. Using rhodococcus erythropolis HL PM-1 as a positive control,the strain L.brevis can degrade the o-Nitrophenol with a high efficiency.In laboratorial condition,L.brevis can degrade 86.7% of the o-Nitrophenol in MMY biodegrading culture medium.The cultured medium was inspected using UV-visible light spectrometer and Mass spectra,and the metabolize route of L.brevis degrading o-Nitrophenol was speculated.更多还原