【摘要】 探讨小鼠胚胎中期肝发育过程细胞增殖与凋亡的变化规律、相关基因 P2 1及 Ki- ras在肝发育中的表达意义。在胚胎中期的小鼠肝组织中 ,采用免疫组织化学及切口末端标记法 ,可检测到小鼠肝发育过程中的细胞增殖与凋亡相伴存在 ,这与肝发育变化密切相关。用原位杂交的方法检测到 P2 1时 ,Ki- ras基因在胚胎肝发育中期并没有表达 ,说明这两个细胞周期调控基因可能在胚胎肝发育中期并不起作用 ,而与其他的基因有关更多还原

【Abstract】 This study is to explore the dynamic trends of cell proliferation, apoptosis and expression of the significance of involved genes as P21 and Ki ras in developing livers of mouse. Lung tissues were taken from different developmental phases of kunming mouse. Immuno histochemistry and TUNEL techniques were adopted to observe the variation of cell proliferation and apoptosis and the expression of characteristics of P21 and Ki ras. Experiments show that cell proliferation is accompanied by apoptosis during the development of mouse livers. The dynamic changes of both ratios might be correlated to the different structures appearing in different developmental process.更多还原